The role of microplastics in the process of Paraclostridium sp. DLY7-assisted phytoremediation of phenanthrene contaminated soil

Abstract

Microplastics can absorb polycyclic aromatic hydrocarbons (PAHs), decrease the bioavailability of pollutants, alter the soil microbial community, and thus might affect the dissipation of soil pollutants. However, whether microplastics could alter the remediation efficiency of PGPR-assisted plants for PAHs-contaminated soil has not been studied. This study assessed the remediation potential of PGPR exhibiting phenanthrene (PHE) degradation ability in assisting maize for PHE-contaminated soil. Especially, the role of microplastics involved in this remediation process was also investigated. The results showed that strain Paraclostridium sp. DLY7 (DLY7) significantly increased the PHE removal efficiency (63.4%) from soil by maize and reduced the content of PHE in maize leaves and roots with no microplastics addition. However, the presence of microplastics significantly inhibited the PHE removal from soil and increased the PHE accumulation in maize issues, in comparison with PHE + DLY7 treatment. Microplastics also significantly reduced soil sucrase and alkaline phosphatase activities, and the content of soil nitrate nitrogen and ammonium nitrogen under non-inoculation with strain conditions. Inoculation with strain DLY7 also altered the rhizosphere soil microbial community, enriched some beneficial bacteria and increased activities of some crucial soil enzymes, thus facilitating PHE removal from soil. On the whole, compared with conventional soil contamination remediation studies, this study innovatively pointed out that microplastics significantly reduced the removal efficiency of soil PHE and increased the uptake of PHE by maize in the PGPR-assisted phytoremediation process. However, the effects of microplastics on microorganisms might be related to the particle size, concentration and type of microplastics in the environment, which would be studied in our future research. These findings proposed new perspectives for revealing the role of microplastics on the efficiency of microbe-assisted phytoremediation of PAHs-contaminated soil.