The Role of Long Non-Coding RNA LINC00467 in Gastric Carcinoma: High Proliferation, Migration and Autophagy

Abstract

The goal of this study is to investigate the role of long non-coding RNA LINC00467 in gastric carcinoma (GC) progression. Differential expressions of LINC00467 in GC samples were detected. By analyzing the follow-up data of recruited GC patients, the influence of LINC00467 on their prognosis was assessed. After knockdown of LINC00467 in SGC7901 and MKN45 cells, cell autophagy was examined by GFP-LC3 puncta assay and Western blot analyses on autophagy-associated genes. Additionally, cell growth and migration were assessed using cell counting kit-8 (CCK-8), 5-ethynyl-2′-deoxyuridine (EdU), and transwell assays. The connection between LINC00467 and miR-18a-5p was studied through a luciferase assay and Pearson correlation analysis. Increased expression of LINC00467 was observed in GC samples and its elevated levels predicted a poor outcome for GC patients. The suppression of LINC00467 reduced the proliferative and migratory capacities of SGC7901 and MKN45 cells. LINC00467 level was closely linked to autophagy activity in GC. The level of miR-18a-5p was decreased in GC tissues and had an inverse correlation with LINC00467, which was established as the target of miR-18a-5p. Upregulated LINC00467 induces growth, metastasis and autophagy activity in GC through negatively regulating miR-18a-5p level, thus leading to the poor prognosis in GC patients.