Abstract
Porphyromonas gingivalis (P. gingivalis) constitutes an essential part of the subgingival dental plaque biofilm, serving as a significant factor in the development of periodontitis. Therefore, establishing a rapid and highly sensitive detection method for P. gingivalis is crucial to effectively manage periodontitis and its associated complications. In this study, droplet digital PCR (ddPCR) technology was employed for the detection of P. gingivalis, with a detection limit of 101 CFU/mL, exhibiting a 10-fold higher sensitivity compared to qPCR (with a sensitivity of 102 CFU/mL). Furthermore, no cross-reactivity was observed with four other bacterial species. In comparison to real-time quantitative PCR, ddPCR demonstrated enhanced sensitivity in detecting P. gingivalis at lower concentrations in 16 simulated samples, indicating its applicability for rapid detection of P. gingivalis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
