Abstract
Objective: The treatment of chronic wounds is one of the actual problems in medicine. Photobiomodulation (PBM) has gained considerable recognition among treatment modalities for various medical problems including wound repair processes. This research aims to investigate the role of intercellular mediators in the regulation of reparative processes of chronic wounds with the PBM therapy in the experiment. Methods: Studies were performed on 12 Wistar rats weighing 250 ± 30g. Rats were used for modeling a chronic wound. Animals were divided into two groups: control and experimental.The animal wounds from the experimental group were treated with low-intensity laser radiation from “Lika-therapist М” in continuous mode using a wavelength of 660 nm, output power at 50 mWt, energy density at 1 J/cm² once per day for 5 days. On the 14th day after the application of wounds, 6 rats from each group were removed from the experiment. PBM efficiency was evaluated with intercellular mediators analysis, as well as with histological examination. ELISA serum analysis was done for quantifying interleukin- 1β (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10) and granulocyte macrophage colony stimulating factor (GM-CSF). Statistical processing of the results was performed using the Statistica 6.0 analysis package. Result: PBM effect on the expression of intercellular mediators in blood serum of animals with chronic wounds after 14 days after wound modeling, showed: increase of cytokine IL-1β (p > 0,05) and IL-4 (p < 0,05) levels; decrease of TNF-α and IL-10 levels (p < 0,05); without significant changes were concentrations IL-6 and GM-CSF. The histological study showed decrease vascular count and better organization of collagen fibers in experimental group. Conclusion: Histological studies of wound healing revealed an earlier transition of the process to the remodeling phase with the use of PBM therapy. PBM therapy can be an instrument for optimizing the reparative process by correcting the regulation by intercellular mediators. Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 398-403
Highlights
Wound healing is a complex, dynamic, biological process that includes the coordinated actions of various types of cells, carried out and regulated by numerous growth factors and cytokines[1]
The concentrations of intercellular mediators in the blood serum of animals whose experimental wounds were exposed to low-intensity laser radiation compared with animals that did not receive photobiomodulation therapy (PBM) therapy are presented in the table
We studied the concentrations of intercellular mediators at the stage of transition of the proliferative phase to the remodeling phase
Summary
Wound healing is a complex, dynamic, biological process that includes the coordinated actions of various types of cells, carried out and regulated by numerous growth factors and cytokines[1]. Given the high prevalence of long-term non-healing wounds[4], increased antibiotic resistance and economic treatment costs[3], it became necessary to find new approaches to stimulate the reparative process. Methods such as microcurrent dressing, application of magnetic surgical tools, treatment with mild vacuum[5,6,7] are being put into practice. As a result of this, the efficiency and safety of using low-intensity laser radiation in medicine do not correspond to the optimal sets of action characteristics (for example, wavelength and radiation dose) and individual characteristics of the patient This makes it difficult to predict treatment outcomes for patients with chronic wounds
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