Abstract
Currently, wound treatment is an urgent task of medicine around the world. In the process of wound healing, various types of cells are involved under the control and regulation of cytokines and growth factors. Disruption of the synchronization process between the various types of cells and intercellular mediators involved in the restoration of tissue damage can lead to impaired healing and the development of chronic wounds. Photobiomodulation (PBM) therapy promotes platelet activation and aggregation, reduces inflammation and oxidative stress, and accelerates cell migration and proliferation. PBM also induces the production of the extracellular matrix and the release of key growth factors, thereby improving tissue regeneration and accelerating wound healing. The aim of our work was to study the effect of photobiomodulation therapy on the regulation of reparative processes in chronic wounds monitored by biomarkers and platelet aggregation activity. 54 Wistar rats were divided into three groups. Intact animals were not manipulated. In animals of the control and experimental groups, a chronic wound was simulated by reproducing the conditions of local hypoxia and microcirculation disorders. The wounds of the experimental group received PBM therapy. The device Lika-therapist M (Ukraine) was used in a continuous mode at a wavelength of 660 nm, an output power of 10 mW, and an energy density of 1 J/cm2. The wounds of the animals in the control group were treated with sham. The animals were euthanized on days 3, 7, 14, and 28 after the surgery (6 animals, each from the control and experimental groups). Measurements of the levels of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-α), the basic fibroblast growth factor (bFGF), and reactive oxygen species (ROS) were carried out by ELISA. Results revealed the multidirectional effect of PBM therapy on the expression of the studied biomarkers. The results of the histological examination indicated a positive effect of PBM therapy with the applied parameters on the repair processes of chronic wounds. We concluded that the use of PBM therapy made it possible to regulate disturbances in reparative processes by modulating ROS, cytokines, and platelet aggregation activity.
Highlights
The theoretical foundations of the possibility of creating a laser were laid by Albert Einstein, who first touched upon the possibility of stimulated radiation in a 1917 article
The aim of our work was to study the effect of photobiomodulation therapy on the regulation of reparative processes in chronic wounds using biomarkers (for example, interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-α), the basic fibroblast growth factor, and reactive oxygen species (ROS)) and platelet aggregation activity
We investigated the effect of PBM therapy, applied in the first 5 days after wound modeling, on the reparation processes of chronic wounds at all stages of wound healing, including the long-term effects of this method on days 14 and 28
Summary
The theoretical foundations of the possibility of creating a laser were laid by Albert Einstein, who first touched upon the possibility of stimulated radiation in a 1917 article. In 1960, the revolutionary discovery of the laser by Theodore Maiman changed the world. Maiman presented his invention to the scientific community in an article in the journal Nature, which contained just under 300 words. Photobiomodulation (PBM) therapy has found applications in the treatment of chronic wounds and in the treatment of many diseases, including neurological and musculoskeletal [2].
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