The role of hydrogen sulfide and carbon monoxide in collagen-induced platelet aggregation in patients with coronary artery disease

S A Belyaeva,O A Trubacheva

doi:10.29001/2073-8552-2020-35-3-86-92

Abstract

Purpose of research. To study the effect of hydrogen sulfide and carbon monoxide donors on collagen-induced platelet aggregation in healthy donors and patients with coronary artery disease. Introduction. There is evidence that the hydrogen sulfide (H 2 S) and carbon monoxide (CO) gas transmitters play an essential role in regulating the functional activity of various organs and systems of the human body. According to previous reports, CO and H S are considered inhibitors of platelet aggregation. Material and Methods . A total of 37 participants were examined during the study. The control group included 13 healthy donors; group of patients with coronary artery disease comprised 24 people. All examined patients received regular combination baseline therapy in accordance with modern recommendations for the treatment of coronary artery disease. Platelet aggregation activity was studied by a turbidimetric method using a two-channel laser analyzer (220 LA Biola, Russia). The degree and rate of platelet aggregation were determined in platelet-rich plasma based on the curves of light transmission and the average size of aggregates as markers of the functional activity of platelets. Collagen at a final concentration of 2 mg/mL was used to induce platelet aggregation. Sodium hydrosulfide (NaHS) was used as a hydrogen sulfide donor. Carbon monoxide-releasing molecule-II (CORM-II) was used as a carbon monoxide donor. Experimental data are presented as Me ( Q 1 – Q 3 ). Wilcoxon T-test was used to test the homogeneity of paired or dependent samples. Values were considered tatistically significant when p was < 0.05. Results. A hydrogen sulfide donor causes a decrease in collagen-induced platelet aggregation along the light transmission curve in both healthy donors and patients with CHD. Collagen-induced platelet aggregation along the light transmission curve is reduced by the action of the carbon monoxide donor in healthy donors, the effect is more pronounced than in patients with CHD. Conclusion. The study showed that the effects of gas transmitters, donors of hydrogen sulfide and carbon monoxide, on the collagen-dependent platelet aggregation were unidirectional and were associated with a decrease in platelet aggregation activity in healthy donors and in patients with coronary artery disease

Highlights

There is evidence that the hydrogen sulfide (H2S) and carbon monoxide (CO) gas transmitters play an essential role in regulating the functional activity of various organs and systems of the human body
Note: * – p < 0.05 compared to the values of collagen-induced aggregation without modifiers; I – degree of platelet aggregation based on the average aggregate size curve, relative units; II – degree of platelet aggregation based on the light transmission curve, %; III – speed of platelet aggregation based on the average aggregate size curve, relative units/min; IV – speed of platelet aggregation based on the light transmission curve, %/min
Note: * – p < 0.05 compared to the values of collagen-induced aggregation without modifiers; I – degree of platelet aggregation based on the average aggregate size curve, relative units; II – degree of platelet aggregation based on the light transmission curve, %; III – speed of platelet aggregation based on the average aggregate size curve, relative units/min; IV –speed of platelet aggregation based on the light transmission curve, %/min

Summary

Материал и методы

В ходе выполнения которого обследованы 37 человек. Результаты и обсуждение Установлено, что у пациентов с ИБС при использовании всех концентраций донора H2S статистически значимо снижалась коллаген-индуцированная агрегация тромбоцитов по кривой светопропускания при концентрации 10-5 М. При значениях концентрации донора H2S 10-4 , 10-5 , 10-6 М скорость агрегации тромбоцитов статистически значимо снижалась по кривой среднего размера агрегатов у пациентов с ИБС Скорость агрегации по кривой светопропускания статистически значимо уменьшалась при действии донора H2S в концентрации 10–6 М. При значении концентрации донора H2S 10–4 М статистически значимо снижалась агрегация по кривой среднего размера агрегатов. Скорость агрегации по кривой среднего размера агрегатов статистически значимо уменьшалась при концентрациях 10–5 и 10–6 М 1. Влияние донора сероводорода на агрегацию тромбоцитов здоровых доноров и пациентов с ишемической болезнью сердца по кривой светопропускания (%) Fig 1. The effect of hydrogen sulfide donor on collagen-induced platelet aggregation in patients with coronary heart disease

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