Abstract
The aim of the study was to investigate the effect of the carbon monoxide (CO) donor on the Ca 2+ -activated potassium permeability of the erythrocyte membrane and platelet aggregation ability. Materials and methods. Healthy volunteers ( n = 27) and patients with chronic coronary heart disease (CHD) ( n = 32) of both sexes were examined. The material of the study was packed red blood cells and platelet-rich plasma obtained from patient’s venous blood. The change of Ca 2+ -dependent potassium conductivity of the erythrocyte membrane was evaluated by potentiometric method, and the platelet aggregation was studied by turbidimetric method. Carbon monoxide releasing molecule-2 ( CORM-2) was used as a CO donor. The amplitude of A23187- and redox-induced hyperpolarization response (HR) of erythrocytes, and the rate and degree of platelet aggregation were estimated. Results. It was shown that the addition of CORM-2 (10 and 100 μM) in the erythrocyte suspension caused a dose-dependent decrease in the amplitude of A23187- and redox-dependent HR in healthy donors, as well as in patients with chronic CHD. The maximum decrease was observed in the presence of 100 μM CORM-2. The effect of CORM-2 at concentrations of 10 and 100 μM on collagen-induced platelet aggregation led to a decrease in the degree and rate of aggregation in healthy donors. The maximum effect was shown at 100 μM of CO donor. However, such an unambiguous effect of CORM-2 on the aggregation parameters in patients with CHD was not observed. Conclusion . The results suggest that CO has a significant effect on the ion transport function of the erythrocyte membrane and platelet aggregation activity of both healthy donors and patients with CHD.
Highlights
The aim of the study was to investigate the effect of the carbon monoxide (CO) donor on the Ca2+-activated potassium permeability of the erythrocyte membrane and platelet aggregation ability
The change of Ca2+-dependent potassium conductivity of the erythrocyte membrane was evaluated by potentiometric method, and the platelet aggregation was studied by turbidimetric method
It was shown that the addition of Carbon monoxide releasing molecule-2 (CORM-2) (10 and 100 μM) in the erythrocyte suspension caused a dose-dependent decrease in the amplitude of A23187- and redox-dependent hyperpolarization response (HR) in healthy donors, as well as in patients with chronic coronary heart disease (CHD)
Summary
Цель исследования – изучить влияние донора монооксида углерода (СО) на Са2+-зависимую калиевую проницаемость мембраны эритроцитов и агрегационную способность тромбоцитов. Потенциометрическим методом изучали изменение Са2+-зависимой калиевой проводимости мембраны эритроцитов, турбидиметрическим методом – агрегационную активность тромбоцитов при действии донора СО (CORM-2). В присутствии 10 и 100 мкМ CORM-2 амплитуда А23187- и редокс-зависимого ГО здоровых доноров, как и пациентов с хронической формой ИБС, дозозависимо уменьшалась, причем максимальное снижение отмечено в присутствии 100 мкМ донора СО. Воздействие CORM2 в концентрациях 10 и 100 мкМ на коллаген-индуцированную агрегацию тромбоцитов приводило к снижению степени и скорости агрегации у здоровых доноров, достигая максимального эффекта при 100 мкМ донора СО. Полученные результаты указывают, что СО оказывает существенное влияние на ион-транспортную функцию мембраны эритроцитов и агрегационную активность тромбоцитов как здоровых доноров, так и пациентов с ИБС. Исследование одобрено локальным этическим комитетом СибГМУ (протокол No 4340 от 30.11.2015)
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