Abstract
The aim of the study was to characterize the features of the subpopulation composition and cytokine-secretory activity of T lymphocytes (Th1, Th17 and Treg) in relation to the concentration of galectin-1 and galectin-3 in the blood of patients with colon cancer. Materials and methods. A total of 26 patients diagnosed with colon cancer were examined. The study material included whole peripheral blood, blood plasma, and supernatants of suspension cultures of mononuclear leukocytes. Lymphocytes isolated from blood were typed by flow cytometry using monoclonal antibodies. The content of galectin-1 and galectin-3 (in blood plasma) and IFNγ, IL-17A, and TGFβ (in supernatants of mononuclear leukocyte culture in vitro ) were determined by enzyme-linked immunosorbent assay. The results obtained were analyzed by statistical methods. Results. In patients with colon cancer, a significant increase in the concentration of galectin-1 and galectin-3 in the blood plasma was found, which was associated with a decrease in the content of CD4+T-bet+ Th1 lymphocytes, CD4+RORC2+ Th17 lymphocytes in the blood and in vitro hyposecretion of IL-17. At the same time, positive correlations were revealed between the concentration of galectin-1 and galectin-3, the content of CD4+FoxP3+ Treg cells in the blood, and the secretion of TGFβ by mononuclear leukocytes in vitro . Conclusion. In colon cancer, increased levels of galectin-1 and galectin-3 in the blood are associated with quantitative deficiency and inhibited secretory activity of effector T lymphocytes and activation of the immunosuppressive functions of regulatory T cells. These results suggest a negative role of galectin 1 and galectin 3 in the mechanisms of regulation of the T cell immune response in colon cancer.
Highlights
The aim of the study was to characterize the features of the subpopulation composition and cytokine-secretory activity of T lymphocytes (Th1, Th17 and Treg) in relation to the concentration of galectin-1 and galectin-3 in the blood of patients with colon cancer
In patients with colon cancer, a significant increase in the concentration of galectin-1 and galectin-3 in the blood plasma was found, which was associated with a decrease in the content of CD4+T-bet+ Th1 lymphocytes, CD4+RORC2+ Th17 lymphocytes in the blood and in vitro hyposecretion of IL-17
Positive correlations were revealed between the concentration of galectin-1 and galectin-3, the content of CD4+FoxP3+ Treg cells in the blood, and the secretion of TGFβ by mononuclear leukocytes in vitro
Summary
Роль галектина-1, -3 в механизмах дисрегуляции Т-клеточного звена иммунного ответа при раке толстого кишечника. Цель исследования – охарактеризовать особенности субпопуляционного состава и цитокин-секреторной активности Т-лимфоцитов (Th1, Th17 и Treg) во взаимосвязи с концентрацией галектина-1 и галектина-3 в крови у больных раком толстого кишечника. Обследованы 26 пациентов (14 мужчин и 12 женщин, средний возраст (62,9 ± 6,7) лет) с диагнозом рака толстого кишечника. Методом иммуноферментного анализа определяли содержание галектина-1 и галектина-3 (в плазме крови) и IFNγ, IL-17A и TGFβ (в супернатантах культуры мононуклеарных лейкоцитов in vitro). При раке толстого кишечника повышенный уровень галектинов 1 и 3 в крови сопряжен с количественным дефицитом и угнетением секреторной активности эффекторных Т-лимфоцитов, и, напротив, активацией иммуносупрессорных функций регуляторных Т-клеток. Полученные результаты указывают на негативную роль галектина-1 и галектина-3 в механизмах регуляции Т-клеточного звена иммунного ответа при раке толстого кишечника. Исследование выполнено при финансовой поддержке Совета по грантам Президента Российской Федерации для государственной поддержки молодых российских ученых (МД2788.2019.7)
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