Abstract
Earlier we reported that mice responded with an acute antinociceptive effect during an 11‐min treatment with HBO2 at 3.5 atmospheres absolute (ATA) (Ohgami et al., NeuroReport 20:1325, 2009). We endeavored to explore the possible role of GABA in this HBO2‐induced effect by pretreating different groups of male NIH Swiss mice, 20‐30 g, intracerebroventricularly (i.c.v.) or intrathecally (i.t.) with various drugs that influenced GABA neurotransmission. The pretreatment drugs were the GABAA antagonist SR 95531, the GABAB antagonist CGP 35348 and the GABA neuronal uptake‐inhibitor (±)‐nipecotic acid. Antinociceptive responsiveness to HBO2 was assessed using the acetic acid‐induced abdominal constriction test conducted in a small animal hyperbaric chamber. HBO2 at 3.5 ATA evoked a strong antinociceptive effect that was antagonized in dose‐related manner by i.c.v.‐ or i.t.‐administered SR 95531. I.c.v. and i.t. pretreatment with CGP 35348 or nipecotic acid potentiated HBO2‐induced antinociception at higher doses. These data strongly suggest that GABA is involved in the acute antinociceptive response of mice to HBO2.Grant Funding Source: Supported by NIH Grant AT‐007222 and the Allen I. White Distinguished Professorship.
Published Version
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