The role of dermatopontin in cell adhesion in bovine placenta during early-mid pregnancy and parturition – Pilot study

Abstract

Dermatopontin (DPT) is a small protein molecule thought to have a role in the formation of the extracellular architecture and adhesion. The aim of the study was to confirm the presence of DPT and to examine its role in placental cell adhesion during pregnancy, at parturition and postpartum in cows.Placental tissue samples were obtained at abattoir from healthy pregnant cows (n = 6) while parturient samples were collected during caesarian section and retrospectively divided into released up to 6 h (R; n = 5) and not released up to 6 h (NR; n = 4) foetal membranes. Maternal epithelial cells were isolated from pregnant samples and were used for the examination of the influence of DPT (5, 50 and 100 ng/mL) on cell adhesion. Parturient samples were manually divided into maternal and foetal part and individually homogenized for Western blotting and ELISA analysis.Western blotting confirmed the presence of DPT in examined tissues. ELISA test showed significant (p < 0.05) decrease in DPT concentration within examined pregnancy period with higher concentrations in maternal part (p < 0.05). Moreover, at parturition DPT concentration further decreased in maternal (p < 0.05) but increased (p < 0.05) in fetal part. The examination of not released samples showed opposite relationship in comparison to parturient samples – the increase in maternal (p < 0.05) and the decrease in fetal (p < 0.05) part of placenta. DPT facilitated the adhesion of epithelial cells in examined periods of pregnancy in increasing manner with pregnancy course.The presence of DPT in bovine placenta during pregnancy and parturition was confirmed. This protein may influence cell adhesion during attachment and detachment of placenta. Further studies on mechanisms of action of DPT in bovine placenta are necessary.