Preliminary results of the placental decorin profile in bovine pregnancy and parturition

Monika Franczyk,Jacek Wawrzykowski,Marta Kankofer

doi:10.1007/s10719-018-9834-7

Monika Franczyk, Jacek Wawrzykowski + Show 1 more

Open Access

https://doi.org/10.1007/s10719-018-9834-7

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Journal: Glycoconjugate Journal	Publication Date: Jul 19, 2018
Citations: 11	License type: open-access

Affiliation: University of Life Sciences in Lublin

Abstract

Decorin is a small leucine-rich proteoglycan which is involved in multiple biological functions mainly as a structural and signaling molecule. Due to its biological properties in connective tissue, decorin may participate in remodeling of ECM during attachment and detachment of placenta within the course of pregnancy and at parturition in cows. The aim of the study was to detect the presence of decorin protein in bovine placental tissues and to evaluate its profile during pregnancy and at parturition. Placental tissues from healthy pregnant cows (2–5 month) were collected in abattoir (n = 10), while parturient tissues were obtained during caesarian section at physiological term (n = 6). Maternal and fetal parts were separated manually and subjected to homogenization and to quantitative ELISA and verification by Western blotting with anti-decorin antibodies. ELISA test showed that the concentration of decorin during pregnancy was higher in the fetal part of placenta compared with the maternal part (p < 0.001). Similar pattern was noted regarding to maternal and fetal samples derived from parturient cows. Our preliminary results demonstrate that the concentration of decorin is gestation time-dependent in healthy bovine placenta. Possible confirmation of the involvement of decorin in early pregnancy attachment and detachment of the placenta during parturition requires further research.

Highlights

The regulation of cell-cell and cell-extracellular matrix adhesion might be involved in both appropriate attachment and detachment of placenta during early pregnancy and labour
One of representatives is decorin (DCN; UniProtKB/Swiss-Prot: P21793; MW of protein core: 39.879 Da; length: 360 aa; pI: 8.72), which is a small leucine-rich proteoglycan first time isolated from bovine placenta in 2000 using chromatographic methods [4]
In our study we focus on the investigation of decorin protein concentration in healthy placentas derived from early pregnancy and at term

Summary

Introduction

The regulation of cell-cell and cell-extracellular matrix adhesion might be involved in both appropriate attachment and detachment of placenta during early pregnancy and labour. In most respects, these adhesive processes may include an interplay between extracellular matrix (ECM) proteins. The ECM, which fills the intercellular space and provides an appropriate environment for animal cells and regulates cell functions through signaling, proliferation, migration and invasion, consists of several simple proteins and proteoglycans [1]. Notwithstanding, apart from these structural proteins there are several biologically active proteins, which are conjugated with sugar moieties. DCN is involved in multiple cellular functions such as proliferation, migration and invasion and acts as a structural molecule, as well as a ligand for receptors [5, 6]

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