Abstract

Summary The effect of a cytokinin, 6-isopentenylaminopurine, on the chromatin-bound RNA polymerase of excised, dark-incubated senescing barley leaves was investigated. During the course of 3 days of dark incubation, the enzyme activity was distinctly higher in the cytokinin-treated material than in that not having been treated. The increased activity was due primarily to the polymerase I, as judged by the isolation method employed and α-amanitin resistance. The relationship between the actions of cytokinin on the polymerase activity and on leaf senescence is discussed.

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