The role of cyclin-dependent kinases in miR-193a5p regulating ovarian cancer cell proliferation and epithelial mesenchymal transformation

Abstract

Objective: To investigate whether miR-193a-5p targets CDK14 and regulates the proliferation and epithelial-mesenchymal transition (EMT) of ovarian cancer cell line OVAC. Methods: TargetScanHuman was used to analyze the match between miR-193a-5p and CDK14, and then miRNA assay was used to detect whether miR-193a-5p targeting CDK14; miR-193a-5p mimics overexpression or miR-193a-5p inhibitor knockdown in the case of low miR-193a-5p, the expression levels of CDK14, EMT-related proteins E-cadherin, vimentin, fibronectin and N-cadherin were detected by immunoblotting, and the proliferation of ovarian cancer cells OVAC was detected by CCK-8, and the cell viability of cancer cell OVAC was detected by MMT. Results: miR-193a-5p targeted the 3'UTR of CDK14; after overexpression of miR-193a-5, the expression of CDK14 was decreased, the expression of EMT-related protein E-cadherin was increased, and the expressions of vimentin, fibronectin and N-cadherin were decreased. The proliferation and cell viability of ovarian cancer cell line OVAC were increased. Meanwhile, after knocking down miR-193a-5p, the expression of CDK14 was increased, and the expression of EMT-related protein E-cadherin was decreased, while the expression levels of vimentin, fibronectin and N-cadherin were increased, and the proliferation and cell viability of ovarian cancer cell line OVAC were decreased. Conclusion: miR-193a-5p reduces the proliferation, cell viability and EMT of ovarian cancer cell line OVAC by targeting the 3 'UTR of CDK14.