The role of adding Hyaluronic Acid in the grafting process for the repair of experimentally induced tibial defect in dogs model

Abstract

The study was designed to determine the role of adding hyaluronic acid as a supportive filling material to improve grafting process of experimentally defective tibial bone in dog model. Eighteen local breed dogs of both sexes weighted 18± 0.4 kg and aged (12± 0.6Months) were randomly allocated in two equal groups. In the first group, twelve dogs underwent experimental tibial bone defect (2.5×0.7 cm ) and replaced with deprotenized lamb ribs. The second group were treated as that for the first, but supported by adding 1% hyaluronic acid, to their grafts. The bone in both groups was firmly fixed by cerclage wire. All dogs were; followed-up clinically, radiologically and macroscopically at (14, 30 and 60 days after the intervention. In all study dogs the systemic infections, signs of seromas, hematoma and sever lameness were not developed along the period of the study . Grossly, dogs in the second group at 60 days showed an increase in the size of the filler of bone at the edges and in the mid of the defect, improvement and acceleration in the bone healing, and formation of bone bridges compared to the first group. Radiological finding exhibited complete healing of the fixated bone segment with the surrounding area; however, some cortical irregularities denoted chronic periosteal reaction and callus formation in the treatment with hyaluronic acid. In conclusion, the study indicated that hyaluronic acid with xenograft materials exhibited remarkable beneficial effects on improvement of the tibial bone defect augmentation