The role of A1 adenosine receptor in pinacidil pretreatment-induced activation of PKCα and ε and protection of vascular reactivity and calcium sensitivity after hemorrhagic shock in rats

Abstract

Objective To observe the role of adenosine receptor in the pinacidil pretreatment-induced activation of protein kinase Cα ( PKCα),protein kinase Ce (PKCe) and protection of vascular reactivity and calcium sensitivity after hemorrhagic shock in rats.Methods The changes of adenosine concentration after pinacidil pretreatment,the effects of A1,A2A,A2B and A3 adenosine receptors (A1 R,A2A R,A2BR and A3 R) inhibitors on the pinacidil pretreatment-induced protein expression and translocation of PKCα and PKCe,and the effects of A1R,A2AR,A2BR and A3R inhibitors on the pinacidil pretreatmentinduced protection of vascular reactivity and calcium sensitivity after hemorrhagic shock were measured.Results Pinacidil pretreatment implemented at 30 min before shock could increase the adenosine concentration at 2 h after shock from 7.299 to 11.626 mg/L ( P ＜ 0.01 ); Pinacidil pretreatment could promote the translocation of PKCα and PKCe from cytoplasm to membrane (P ＜0.01 ),and induce the protection of vascular reactivity and calcium sensitivity after hemorrhagic shock,which were all inhibited by A1 R inhibitor ( P ＜ 0.01 ).Conclusion Pinacidil pretreatment could induce the activation of PKCα,PKCe and the protection of vascular reactivity and calcium sensitivity after hemorrhagic shock in rats through A1 R. Key words: Pinacidil; Pretreatment; Vascular reactivity; Calcium sensitivity