The road map to proper dental pulp experiments in animal models

Abstract

Animal models are essential for the development and comparison of new dental materials. However, working on the dental pulp of animal models can be challenging. To provide guidance for conducting proper dental pulp experiments in animal models. The author conducted a literature search on the Google Scholar database to find papers related to pulp capping and animal modeling in dentistry. Based on her own experience in animal research and the findings from the literature review, the author provides practical advice for new researchers in this field. Dogs and monkeys have the size and morphology of teeth that facilitate accessibility while operating in the dental pulp. However, the response of rat dental pulp has been found to be identical to that of humans. Rat maxillary molars are often used for dental pulp studies. Anesthesia using a combination of ketamine and xylazine provides a suitable time for pulp procedures in rats, but it's important to note that anesthetized rats may remain alert despite receiving the correct dose. Attempting to increase the dosage can result in the rat being unable to recover from anesthesia. To ensure safe and effective anesthesia, it is recommended to administer only one-third of the ketamine dose. Xylazine re-dosing should be avoided. Dental researchers in the Middle East and Africa may face unique challenges, but working on rats is challenging worldwide. In the future, virtual reality and simulation may offer alternatives for experimental animals. Wister Albino is the more suitable animal model to be used in dental pulp experiments. The checklist and flow chart of PRAISE 2021 are mandatory for dental researchers experimenting with the dental pulp of animal models.