Abstract
Plant lipoxygenases (LOXs), a kind of non-heme iron-containing dioxygenases, participate plant physiological activities (especially in response to biotic and abiotic stresses) through oxidizing various lipids. However, there was few investigations on LOXs in foxtail millet (Setaria italica). In this study, we identified the LOX gene family in foxtail millet, and divided the total 12 members into three sub-families on the basis of their phylogenetic relationships. Under salt and drought stress, LOX genes showed different expression patterns. Among them, only SiLOX7 showed up-regulated expression in Yugu1 (YG1) and Qinhuang2 (QH2), two stress-tolerant varieties, indicating that SiLOX7 may play an important role in responses to abiotic stress. Our research provides a basis for further investigation of the role of LOX genes in the adaptation to abiotic stresses and other possible biological functions in foxtail millet.
Highlights
Drought and salt are both the main abiotic factors limiting the growth and development of plants, and thereby crop yield [1]
Lipoxygenases catalyze the production of oxylipins, which play a key role during the whole growth period of plants, such as seed germination and plant growth, especially against biotic and abiotic stresses [9,10,11,12]
We identified 12 LOX genes of foxtail millet and analyzed their distribution on chromosomes, encoded protein sequences, phylogenetic relationships, classification, conserved domains and expression patterns and physiological parameters under different abiotic stresses, which is helpful for investigating the molecular mechanism of stress tolerance in foxtail millet, as well as the related studies in other C4 crops
Summary
Drought and salt are both the main abiotic factors limiting the growth and development of plants, and thereby crop yield [1]. Lipoxygenases (oxygen oxidoreductase, EC 1.13.11.12; LOX) belong to non-heme ironcontaining fatty dioxygenases which catalyze the degradation of polyunsaturated fatty acids [4,5]. This kind of iron-containing dioxygenase was widely present in fungi, animals, plants, algae, protozoa and bacteria cells [6,7]. Lipoxygenases catalyze the production of oxylipins, which play a key role during the whole growth period of plants, such as seed germination and plant growth, especially against biotic and abiotic stresses [9,10,11,12]. Rice seeds with reduced LOX enzyme activity showed higher germination efficiency after storage [13]. The metabolites of LOX pathway are the fatty acid oxidation products mediated by LOX, known as oxylipins, such as jasmonates (JA), green leaf volatiles (GLVs) and death acid
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