Abstract
Objective To study the different drying technology on Wudang Lonicerae Flos (Ⅰ) in chlorogenic acid content. Methods Using HPLC method to research the difference in the contents of chlorogenic acid of Wudang Lonicerae Flos (Ⅰ) employing oven-drying method, air-dried method and steamed drying technologies. DIONEX C18 (4.6 mm×250 mm, 5 μm) was used. The mobile phases consisted of acetonitrile-0.4% phosphoric acid aqueous solution (17∶83). The detection wavelengths of chlorogenic acid was 327nm. The flow rate was 0.4 ml/min. The column temperature was 30 ℃. Results The Separation of Chlorogenic acid content was satisfactory. The concentration and peak area showed a good linearity relationship with the range of 0.285-2.850 μg. Precision, repeatability and stability met the requirements. The average recovery was 100.26% with RSD 1.95%. Chlorogenic acid content in the steamed drying Wudang Honeysuckle (Ⅰ) was higher. Conclusions The method of steam drying is better than that of oven-drying method and drying. Key words: Chromatography, high pressure liquid; Flos Lonicerae; Chlorogenic acid; Quality control
Published Version
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