Abstract
Sugar-unspecific nucleases diffuse out of intact and ungerminated pollen grains of Nicotiana tabacum within 2 min of suspension in pollen medium. No further increase in nuclease activity of the medium was detected during the subsequent pollen cultivation. Two fractions of the nuclease activity are separable by diethylaminoethyl-cellulose (DEAE-C) chromatography differing in relative activities towards native DNA, denatured DNA and RNA. The pH optimum for hydrolysis of the different substrates range from 5.0 to 5.7.
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