Abstract
A simple, non-toxic and effective method to separate germinated from ungerminated pollen grains has been developed using pollen from corn ( Zea mays L. cv. Pioneer 3747). After separation, 99.1% of the grains in the sample of germinated pollen grains had tubes; 97.2% of the grains in the sample of ungerminated pollen grains had no tubes. Recovery was 18.1% of the total grains for germinated and 1.5% for ungerminated pollen grains. The separated germinated pollen grains retained viability and continued tube growth when placed in culture medium.
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