Abstract
The possibility that lymphokine-activated killer (LAK) cells versus spontaneous natural killer (NK) cells show relative resistance to the suppressive effects of the immunoregulatory molecules prostaglandin E 2 (PGE 2) and dexamethasone (DMO) was investigated. LAK cells were produced in vitro by the incubation of human peripheral mononuclear cells (PBMC) for three days in the presence of interleukin-2 (IL-2). Cytotoxicity of NK and LAK cells were measured by conventional 4 hour Cr 51 release assays using K562 and Daudi target cells. LAK cells were relatively resistant to suppression by PGE 2. For example, NK cytotoxicity was significantly suppressed by 10 −6 M PGE 2. In contrast, LAK cells required a 30 to 100 higher concentration of PGE 2 according to the target used to achieve similar suppression. Likewise, a differential resistance to DMO was seen. NK cells were significantly suppressed by 10- 3M DMO while a 1000 fold higher concentration was needed for similar suppression of LAK cytotoxicity. Overall, the results show that LAK cells are relatively resistant to immunoregulatory suppressive factors.
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