Monoclonal antibody against TXB 2: Its use in solid and liquid phase radirmmunoassays

Abstract

Monoclonal antibodies to thromboxane B 2 (TXB 2) have been produced and characterized. Both liquid and solid phase radio immunoassays have been developed using one of these monoclonal antibodies. The two assays gave similar results when used to quantitate TXB 2 in 11 serum samples; however the solid phase assay was more sensitive than the liquid phase assay (i.e., 63 pg/ml vs 19 pg/ml) at a B/B 0 = 90%). Despite a difference in the sensitivity of the two assay systems, the cross-reactivity of the monoclonal antibody for PGD 2, PGE 2, PGF 2α and 6 keto-PGF 1α was the same.