The relationships between LncRNA NNT-AS1, CRP, PCT and their interactions and the refractory mycoplasma pneumoniae pneumonia in children

Ping Chen,Lumin Chen,Jianfeng Xie,Hongli Lin,Shihao Zhuang,Zheng Huang,Kuicheng Zheng

doi:10.1038/s41598-021-81853-w

Abstract

To investigate the relationships between LncRNA NNT-AS1, CRP, PCT and their interactions and the refractory mycoplasma pneumoniae pneumonia (RMPP) in children. Serum levels of LncRNA NNT-AS1 of RMPP and non-RMPP (NRMPP) patients were detected by real-time PCR, and were analyzed together with serum c-reactive protein (CRP) and procalcitonin (PCT). Correlations between LncRNA NNT-AS1 and CRP and PCT were analyzed by Pearson correlation test. The ROC curve was used to analyze the potential of LncRNA NNT-AS1, CRP and PCT as biomarkers for predicting RMPP. Logistic regression crossover model and the Excel compiled by Andersson et al. were used to analyze the interactions among the biomarkers. We found that LncRNA NNT-AS1, CRP and PCT were all highly expressed in patients with RMPP. LncRNA NNT-AS1 could positively correlate with the expressions of CRP and PCT, and jointly promote the occurrence of RMPP. The combined diagnosis of LncRNA NNT-AS1, CRP and PCT could predict the occurrence of RMPP.

Highlights

To investigate the relationships between LncRNA NNT-AS1, c-reactive protein (CRP), PCT and their interactions and the refractory mycoplasma pneumoniae pneumonia (RMPP) in children
Blood test results showed that compared with NRMPP children, serum CRP (Fig. 1B) and PCT (Fig. 1C) were significantly increased in RMPP children. These results indicated that the abnormal expressions of LncRNA NNT-AS1, CRP and PCT may be correlated with the occurrence and development of RMPP
In order to further explore whether the expressions of IgA, IgG, CRP and PCT levels are related with LncRNA NNT-AS1, the 150 objects were divided into high expression and low expression groups, each with 75 objects

Summary

Introduction

To investigate the relationships between LncRNA NNT-AS1, CRP, PCT and their interactions and the refractory mycoplasma pneumoniae pneumonia (RMPP) in children. LncRNA NNT-AS1 could positively correlate with the expressions of CRP and PCT, and jointly promote the occurrence of RMPP. Studies have shown that the frequent abnormal expressions of lncRNA in tissues and cells may be closely related to the occurrence and development, invasion and metastasis of tumors and drug resistance[11]. Wang et al.[14] found in combination with in vitro and in vivo experiments that LncRNA NNT-AS1 was highly expressed in colorectal cancer tissues and could promote the proliferation, metastasis and invasion of colorectal cancer cells by regulating the MAPK/ERK signaling pathway. In this study, the expressions of lncRNA NNT-AS1, CRP and PCT were detected and their correlations with RMPP were analyzed to achieve early diagnosis and treatment, shortening the course of the disease and reducing the occurrence of related sequelae

Methods

Results

Conclusion