The relationship of P47phox C923T (Ala308Val) polymorphism to cerebral infarction and its effect on plasma lipid levels

Abstract

To investigate the relationship between C923T (Ala308Val) polymorphism in the exon10 of P47(phox) gene and cerebral infarction and to evaluate the effect of C923T polymorphism on plasma lipid levels. Peripheral blood samples were collected from 110 patients with cerebral infarction, 100 sex and age-matched healthy controls, and 102 members of 10 cerebral infarction pedigrees 19 of which were cerebral infarction patients. The polymorphism in P47(phox) gene was determined by PCR-single strand conformation polymorphism analysis and DNA sequencing. Plasma lipid levels were measured by routine methods. Three gene types, CC, CT, and TT were found in the C923T (Ala308Val) polymorphism site. No statistically significant differences were found in the frequencies of genotypes and alleles of C923T polymorphism between the controls and cerebral infarction patients (all P > 0.05). The serum levels of triglyceride of cerebral infarction patients and controls with the CT genotype were markedly higher than those of the cerebral infarction patients and controls with the CC genotype (both P < 0.05). There is no association between the C923T polymorphism and cerebral infarction. C923Tpolymorphism is associated with plasma lipid metabolism.