Abstract
Objective To explore the relationship between the expression of vascular endothelial growth factor(VEGF)and angiogenesis in glenohumeral of diabetic rats. Methods The 15 specific pathogen free (SPF)male SD rats that weighing between 240 and 260 gram were randomly divided into two groups,namely the model group(10)and the control group(5).After the success of modeling,the rates of both groups were sacrificed, take synovial tissues of rotator cuff from each group, stain and evaluate them.The expression of VEGF was determined by immunohistochemistry and Western blotting.The evalution of vascular density was measured by CD34 immunoreactivity. Results The hyperemia of synovial tissue from rotator cuff was observerd in model group, which showed strong immunoreactivity compared with the synovial tissue in control group, the immunohistochemistry of VEGF was++++(4),+++(4),++(2)compared with the control group++ (4),+(1)(P<0.05), the CD34 immunohistochemical staining showed that the average number of vessels per×400 field of vision in the model group and the control group were(34.1±15.9)and(9.4±4.5)respectively(P<0.05).It also shows that the expression of VEGF was more than the control group by Western blot. Conclusion VEGF is more expressed in the synovial tissue of rotator cuff in diabetic rats, which may play a role in pathological mechanisms and angiogenesis of diabetic rats frozen shoulder. Key words: Diabetes; Frozen shoulder; Synovial; Vascular endothelial growth factor; Angiogenesis
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call