The relationship between the expression of iNOS and COX-2 in oral squamous carcinoma and vascular endothelial growth factor

Abstract

Objective To investigate the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2)protein in oral squamous cell carcinoma tissue,and to explore relationship and vascular endothelial growth factor (VEGF) of them.Methods The expression of iNOS,COX-2 and VEGF in 54 cases of oral mucosa squamous cell carcinoma tissue were detected by immunohistochemistry SP staining.And its correlation to clinicopathologic features were analyzed.Results The positive expression rates of iNOS,COX-2 and VEGF were 66.7％,55.6％,62.5％ respectively.The positive expression of iNOS,COX-2 and VEGF was correlated with pathological grade,TNM stage and tumor recurrence (P ＜ 0.05).There were positive correlations between the expression of iNOS and VEGF,COX-2 and VEGF(P ＜ 0.05).Conclusions The expression of iNOS,COX-2 and VEGF are both higher,they are closely correlated to biological action of oral carcinoma,iNOS,COX-2 participate in VEGF promote the tumor angiogenesis. Key words: Oral squamous cell carcinoma; Carbon monoxide synthase; Cyclooxygenase-2; Vascular endothelial growth factor; Immunohistochemistry