The relationship between MTHFR polymorphisms and abortion in Iranian women

Abstract

BackgroundRecurrent spontaneous abortion (RSA) is a significant obstetrical complication during pregnancy. Two common polymorphisms (C677T and A1298C) of the methylenetetrahydrofolate reductase (MTHFR) gene are risk factors for RSA. This study was performed to determine the effect of C677T and A1298C polymorphisms in RSA. The true relationship between these two mutations and risk of recurrent spontaneous abortion is unknown, and it is unclear if women with these mutations should be given anticoagulants during pregnancy. Materials and methodsIn this prospective and experimental study, one hundred and fifty three individuals were referred with clinical findings that included recurrent spontaneous abortions minimum of 2 and maximum of 5. Total genomic DNA was isolated from peripheral blood samples. To determine the frequency of the two common C677T and A1298C MTHFR gene polymorphisms in the patients, we used PCR-restriction fragment length polymorphism method. ResultsThe frequency of Heterozygous and homozygous C677T polymorphisms were 18.13% and 13.1% respectively. The frequency of Heterozygous and homozygous A1298C polymorphisms were 18.3% and 15.7% respectively. The frequency of Heterozygous C677T and A1298C polymorphisms were 26.8% and wild-type individuals were 7.8%. All female subjects who had heterozygous or homozygous C677T and A1298C polymorphisms with recurrent RSA were followed and those who received appropriate anti-coagulant therapy resulted in successful pregnancy. ConclusionIn present study, we provided witness to support the relationship between MTHFR C677T and A1298C polymorphisms and recurrent spontaneous abortion. Also, we suggest that anticoagulation therapy of these patients during pregnancy could lead to a successful pregnancy outcome.