Abstract
Peroxidase activity in excised stem segments of Pisum sativum L. cv. Progress No. 9 increases linearly during incubation in buffered medium. Gibberellic acid (GA) causes a slight reduction in activity whereas indoleacetic acid (IAA) treatment completely prevents this rise. Excision produces two new cathodic isoperoxidases near the cut ends. Their appearance is prevented by cycloheximide, actinomycin D, and IAA; G A enhances this IAA-induced repression. GA alone stimulates one of the isozymes but does not affect the other one. Peroxidase leakage is stimulated by GA and inhibited by IAA treatment. The activity is entirely confined to the isoperoxidases produced in response to injury.IAA oxidase activity in incubated segments was slightly elevated over that of freshly cut ones. However, differences in activity among hormone treatments were small. IAA oxidase was also demonstrated in growth media and only IAA treatment reduced activity. Both peroxidases in the medium were isolated by column chromatography. Surprisingly, the purified isozymes appear to be essentially devoid of IAA oxidase activity.
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