Abstract

Summary Applied indoleacetic acid (IAA), Giberellic acid (GA) and Ethrel (separate and in combination) to excised apical sections of etiolated Alaska pea ( Pisum sativum ) seedlings indicate that both GA and IAA cause a depression in the activities of peroxidase, catalase and polyphenol oxidase, in comparison to untreated controls. Ethrel treatment cause a significant decrease in the activity of both catalase and polyphenol oxidase vs. untreated control tissue. GA plus Ethrel and IAA plus Ethrel both mediate lowered levels of catalase, peroxidase and polyphenol oxidase activities, in comparison to untreated controls. Ethrel treatment causes a depression in peroxidase activity vs. applied GA or IAA. The present study suggests that excised pea stem tissue segments, immediately below the apical area, respond differentially with respect to the activities of catalase, peroxidase and polyphenol oxidase, when treated with GA or IAA alone, or in combination with Ethrel. However, Ethrel, applied together with either GA or IAA, has a depressor effect on all three respective enzyme activity levels. In all instances, the effects of IAA, GA and Ethrel treatments, alone or in combination, were of a lower order of magnitude than the corresponding untreated control stem sections.

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