Abstract
Objective Collect clinical specimens of gastric cancer patients with metastasis and without metastasis, application of molecular biology method confirm cytochrome B in its role in the development of cancer of the stomach, provide experimental basis for exploring the mechanism of gastric cancer. Methods Collect 12 cases confirmed by pathology of gastric cancer surgery in patients with gastric cancer tissue, including specimens with and without lymph node metastasis specimens each six cases. Detect the expression of two groups of gastric cancer tissue apoptosis related factors B cell lymphoma/leukemia- 2 associated X protein(bax), B cell lymphoma/leukemia- 2(bcl- 2), Caspase and mitogen- activated protein kinase(MAPK)signaling pathway proteins level by Western blotting. Results Compared with the group without metastasis, the expression of promoting apoptosis factor bax was reduced in gastric cancer tissue(0.55:1.00, P< 0.05), and the expression of inhibiting apoptosis factor bcl-2 increased(1.32:1.00, P<0.05), and the level of cell apoptosis decreased(2.86:3.35, P< 0.05). MAPK pathway activation level in gastric cancer tissue with metastasis was significantly reduced by comparing with the gastric cancer tissue without metastasis(P< 0.05). Conclusion Cytochrone B expression level decrease, thus inhibit MAPK pathway phosphorylation and reduce the cell apoptosis. Gastric cancer metastasis is related to the above mechanism. Key words: Gastric cancer; Mitochondrial cytochrome B; Apoptosis; Mitogen-activated protein kinase pathway
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