Abstract
Objective To investigate the expression and function of microRNA (miRNA, miR)-429 in human gastric precancerous lesions. Methods The expression of miR-429 in gastric cancer tissues (n=50), gastric tissues with chronic atrophic gastritis (n=60) and normal gastric tissues (n=60) was detected by real-time quantitative polymerase chain reaction (PCR). Human Gastric Epithelial cell strain-1 (GES-1) was transformed into gastric cancer precancerous lesion (PLGC) cell model by tumor necrosis factor-α (TNF-α). PLGC cells were detected by cell counting kit-8 (CCK-8) method using the miR-429 lentivirus and lentivirus negative control. The target gene of miR-429 was predicted by targetscan, and the expression level of target gene was deteceted by Western blotting. Results The expression of miR-429 in gastric tissue of 54 patients (90%, 54/60) with chronic atrophic gastritis was lower than that of normal gastric tissue, of which 37 cases (61.7%, 37/60) decreased more than 2 times. What’s more, the expression of miR-429 in the chronic atrophic gastric tissues with metaplasia and atypical hyperplasia was statistically much more significant (P=0.012). The expression of miR-429 in all gastric cancer tissues was lower than that in normal gastric tissues and chronic atrophic gastric tissues (all P=0.003), and the expression level was negatively correlated with lymph node metastasis, adjacent vascular invasion and TNM staging. The growth of PLGC cells was significantly inhibited at 5th day after transfection with miR-429, and the absorbance (0.852±0.023) was significantly reduced as compared with the control group (1.488±0.031, P=0.000). The expression of CDC25B in the transfection group (the target gene of miR-429 found by targetscan) was significantly lower than that of control group. Conclusion The expression of miR-429 in the gastric tissue of chronic atrophic gastritis and gastric cancer tissues was significantly down-regulated, and it could inhibit the development of gastric precancerous lesions to gastric cancer and the invasion and metastasis of gastric cancer. CDC25B might be the target gene of miR-429. Key words: Gastric precancerous lesions; Gastric cancer; Chronic atrophic gastritis; MicroRNA; Gene CDC25B
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
