Abstract
A one-phase solvent consisting of n-butanol, methyl ethyl ketone, 17 ammonia and water (5:3:1:1, v/v) has been used instead of phenol in two-dimensional paper chromatography for which n-butanol-acetic acid-water (4:1:5, v/v) is used as the second solvent. Clear separations, allowing quantitative recoveries, of the amino acid constituents of proteins have been obtained. Losses of amino acids during chromatography and subsequent estimations by a ninhydrin colorimetric procedure are small, being mainly from 1–4%. The R F values of 25 naturally occurring amino acids are given for this aqueous butanol-ketone-ammonia solvent.
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