Abstract
1. 1. RNAase from the roe of the scallop, Chylamys opercularis, was purified 192-fold, with an apparent recovery of 97 per cent. The final product was free of DNAase, phosphodiesterase and phosphomonoesterase activities. 2. 2. The enzyme is an acid RNAase, with a pH optimum of 5·2, at I = 0·3. 3. 3. The molecular weight of the enzyme is 38,500. 4. 4. The scallop roe RNAase exhibited no marked specificity towards bonds adjacent to either purine or pyrimidine nucleotides. However, the K m for Poly(A) (2·2 μg/ml) was considerably lower than K m for Poly(U) (400 μg/ml). Neither Poly(C) nor (Poly(G) was hydrolysed by the enzyme.
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