Abstract
Fractionation of 1000 Uca pugilator eyestalks by a combination of gel filtration on Sephadex G-25 and successive ion exchange chromatography on CM- and DEAE-cellulose resulted in the isolation of two major peaks of melanophore-dispersing activity which were designated MDH-1 and MDH-2. These hormones coeluted on Sephadex G-25 superfine, but showed distinct elution profiles on CM-cellulose. The homogeneity of MDH-1 was determined in two different paper chromatography and paper electrophoresis systems. In these systems a single fluorescamine-positive spot comigrated with MDH activity. N-Terminal analysis of MDH-1 by the dansyl-Cl procedure resulted in the identification of aspartic acid at this position. MDH-1 stimulated pigment dispersion in melanophores and leucophores in an identical manner, and in both systems a dose of 30 ng/μl caused a half-maximal response.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
