The proteins induced in black beetle virus-infected Drosophila cells at elevated temperature

Abstract

Summary The virus of black beetle ( Heteronychus arator ) had little effect on the host cell protein synthesis during the early stages of infection of Drosophila melanogaster cells. Raising the culture temperature from 28 to 37° C induced heat shock proteins (hsp) and inhibited the normal pattern of cell protein synthesis. This permitted clearer visualization of 4 virus-specific polypeptides in infected cells. Actinomycin D inhibited hsp production and an additional high molecular weight viral polypeptide became apparent in infected cells.