The Protective Role of Melatonin in Sperm Cryopreservation of Farm Animals and Human: Lessons for Male Fish Cryopreservation.

Abstract

Simple SummaryIn recent years, cryopreservation of fish sperm has been a rapidly evolving technique that contributes both to the improvement of genetic reproduction programs and the proper management of broodstock as well as to ensuring the viability of endangered species. However, this technique can cause significant damage to sperm, making the use of cryoprotectants and antioxidants in cryopreservation solutions imperative. The hormone melatonin has demonstrated positive effects on the cryopreservation of sperm in both farm animals and humans. Therefore, the plethora of research that has been conducted on animals and humans could be expanded to fish cryopreservation, making melatonin potentially a very promising alternative cryoprotectant.Cryopreservation is a technique that offers various advantages, especially in fish, among others, that makes the reproduction of species easier through a constant supply of sperm, synchronization of the gamete availability of both sexes, storage of semen for genetic improvement programs, reduction in the cost by eliminating the need to maintain male broodstock, and conserving the gametes of endangered species. However, freezing and warming procedures for cryopreservation lead to a reduction in the quality and viability of cryopreserved sperm because of oxidative stress. For this reason, the enrichment of extender media with antioxidants is a common method of cryopreservation of the semen of several fish species. Recently, many studies have been published for the protective role of antioxidants and especially of melatonin on male fertility preservation both in farm animals and humans, demonstrating the beneficial effects of melatonin as a sperm cryoprotectant. On the other hand, very few studies were conducted using melatonin as an antioxidant in different male fish species for semen cryopreservation. We conclude that the use of moderate concentrations of melatonin are beneficial to semen preservation, and the mechanisms through which melatonin acts positively on spermatozoa need to be further investigated to establish improvement protocols for cryopreservation in fish species.