Abstract
Objective To study whether the calcium channel blockers can alleviate calcium overload to protect liver function during cold storage. Methods The rat liver cells were preserved hypothermically for different intervals and divided into various groups in terms of preserved solutions: control group with DMEM solution, experimental group Ⅰ with DMEM solution plus Verapamil, experimental group Ⅱ with DMEM solution plus nifedipine, experimental group Ⅲ with DMEM solution plus diltiazem. Fura（2 method was used to measure the intracellular calcium of the rat hepatocytes and liver function during cold storage. The hepatocytic structure was observed under light and electron microscopy. Results There was significant difference between the experimental groups and control group in intracellular calcium concentration and liver function. The histological injury at 0 to 48?h of cold storage in the experimental groups was obviously milder than that in the control group. Conclusion Calcium channel blockers could protect the hypothermically preserved rat liver. Verapamil was the strongest agent of the three blockers.
Published Version
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