Abstract
BackgroundAngelica root is the dry root of the Umbelliferae plant Angelica sinensis (oliv) Diels. Angelica organic acid (OA) is the main active ingredient in Angelica sinensis, and it exerts potential anti-atherosclerotic effects by preventing Oxidized low-density lipoprotein (Ox-LDL) induced endothelial injury. To study the protective effects of OA on ox-LDL-induced HUVECs autophagic flux dysfunction and inflammatory injury.MethodsOA were isolated by water extraction and alcohol precipitation, and then the content of ferulic acid (FA) in the OA was determined by high performance liquid chromatography. The ox-LDL-induced endothelial injury model was established. The effect of ferulic acid on the survival of Human umbilical vein endothelial cells (HVUECs) was detected by CCK-8 assay. HUVECs were pretreated with different concentrations of OA (20 μmol/L, 40 μmol/L, and 80 μmol/L), and Western Blot was used to detect the expressions of LC3II, p62, MCP-1, VCAM-1 and LOX-1. The autophagosomes in HUVECs were observed by transmission electron microscopy (TEM).Results20 μmol/L OA could increase the expression of LC3II and decrease the expression of p62, MCP-1, VCAM-1 and LOX-1. The results of TEM showed that angelica organic acids promoted cell organelle degradation in autolysosomes.ConclusionOA could reduce inflammation, protect endothelial cells and play an anti-atherosclerotic role by enhancing the autophagy flux of damaged endothelial cells, in which FA the major active ingredient of OA played a major role.
Highlights
Angelica root is the dry root of the Umbelliferae plant Angelica sinensis Diels
Analyzing the chromatograms of ferulic acid (FA) in angelica organic acids The test solution of FA and the standard solution had a peak at the same retention time (12.54 min)
Ox-Low density lipoprotein (LDL) induces autophagic flux damage in Human umbilical vein endothelial cells (HUVECs) The results of transmission electron microscopy (TEM) showed that when HUVECs were treated with 20 mg/L ox-LDL, a small amount of autophagosomes appeared in the cytoplasm
Summary
Angelica root is the dry root of the Umbelliferae plant Angelica sinensis (oliv) Diels. Angelica organic acid (OA) is the main active ingredient in Angelica sinensis, and it exerts potential anti-atherosclerotic effects by preventing Oxidized low-density lipoprotein (Ox-LDL) induced endothelial injury. To study the protective effects of OA on ox-LDL-induced HUVECs autophagic flux dysfunction and inflammatory injury. Ox-LDL can lead to the formation and progression of atherosclerotic plaque by inducing activation and dysfunction of endothelial cell, formation of macrophage foam cell, and migration and proliferation of smooth muscle cell [1]. Its main mechanism is to inhibit the expression of endothelial adhesion molecules and reduce oxidative stress and inflammation [7, 8].
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