Abstract
Objective To explore the effects of perfluorocarbon (PFC) on the damaged typeⅡ alveolar epithelial cells (AEC Ⅱ) induced by lipopolysaccharide (LPS), and the apoptosis and inflammatory reaction of AEC Ⅱ induced by LPS. Methods Primary AEC Ⅱ was divided into control group according to the random number table method, LPS group, PFC group and PFC+ LPS group. LPS group: LPS (1 μg/ml) was added to cells. PFC group: PFC (20%) was added to cells. PFC+ LPS group: PFC (20%) and LPS (1 μg/ml) were added to cells. The apoptotic rate of AEC Ⅱ was detected by flow cytometry. Morphologic change was observed by electron microscope. Concentrations of interleukin (IL)-6 and IL-10 of supernatant were detected by ELISA. Results Apoptotic rate of AEC Ⅱ remarkably increased in LPS group than in control grouop [(10.89±1.04)% vs (14.29±1.93)%] (P 0.05). Conclusion PFC can protect AEC Ⅱ against the injury induced by LPS. PFC can also release the level of inflammatory response. Key words: Perfluorocarbon; Alveolar epithelial cell; Lipopolysaccharide; Apoptosis; Inflammatory factor
Published Version
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