The protective effect of perfluorocarbon on the injury of alveolar epithelial cells induced by lipopolysaccharide

Abstract

Objective To explore the effects of perfluorocarbon (PFC) on the damaged typeⅡ alveolar epithelial cells (AEC Ⅱ) induced by lipopolysaccharide (LPS), and the apoptosis and inflammatory reaction of AEC Ⅱ induced by LPS. Methods Primary AEC Ⅱ was divided into control group according to the random number table method, LPS group, PFC group and PFC+ LPS group. LPS group: LPS (1 μg/ml) was added to cells. PFC group: PFC (20%) was added to cells. PFC+ LPS group: PFC (20%) and LPS (1 μg/ml) were added to cells. The apoptotic rate of AEC Ⅱ was detected by flow cytometry. Morphologic change was observed by electron microscope. Concentrations of interleukin (IL)-6 and IL-10 of supernatant were detected by ELISA. Results Apoptotic rate of AEC Ⅱ remarkably increased in LPS group than in control grouop [(10.89±1.04)% vs (14.29±1.93)%] (P 0.05). Conclusion PFC can protect AEC Ⅱ against the injury induced by LPS. PFC can also release the level of inflammatory response. Key words: Perfluorocarbon; Alveolar epithelial cell; Lipopolysaccharide; Apoptosis; Inflammatory factor