Abstract
An important role in the development of acne is played by the activity of the skin microflora (namely the bacteria Propionibacterum acnes) and members of the genus Staphylococcus. In the schemes of complex treatment of patients with this disease, antibiotics from among macrolides or lincosamides are mainly used. It is of interest to study the current state of susceptibility of staphylococci and propionic bacteria to these drugs, as well as to other antimicrobial agents in order to determine the prospects for expanding the arsenal of methods for treating acne. The aim of the study was to study the susceptibility of microorganisms involved in the development of acne to antimicrobials and antagonistic effects of probiotic strains of lactobacilli. We conducted a bacteriological study of the content of rash elements in 60 patients with acne. The biological characteristics of 40 strains of Staphylococcus spp. and 15 strains of Propionibacterum acnes isolated and researched. The sensitivity of microorganisms to antibiotics and antiseptics was determined by the disco-diffusion method and the method of serial double dilutions of drugs in a liquid nutrient medium. Determination of the sensitivity of acne pathogens to the antagonistic effect of lactobacilli was performed by the method of two-layer agar by reducing the number of microorganisms. The results of the study made it possible to establish that the isolated strains of bacteria show a low level of sensitivity to antimicrobials, which in medical practice are most often used for topical therapy of acne. Only 17.5 % of isolated staphylococcal strains were sensitive to erythromycin, only 45.0 % of strains of this species were sensitive to clindamycin. Strains of Propionibacterum acnes showed a higher level of sensitivity to antibiotics than staphylococci. However, the established indicators do not allow to consider highly effective means for treatment of acne on their basis. At the same time, a high level of sensitivity of acne pathogens to antiseptic drugs decamethoxine and Aeroplysinin 1 and antagonistic effect of Lactobacillus acidophilus was established. Thus, the strains of Propionibacterum acnes and Staphylococcus spp., involved in the development of acne, are characterized by high levels of resistance to erythromycin, clindamycin, chloramphenicol, benzoyl peroxide, which are now the basis of the arsenal of topical therapy for patients with this disease. There is a need to find new effective drugs for the etiotropic therapy of acne.
Highlights
In the context of the global spread of the phenomenon of polyresistance of microorganisms to antimicrobials, the urgency of finding treatments for diseases of microbial etiology is constantly growing
Minimum bactericidal (MBC) concentrations of antibiotics most commonly used in the treatment of acne, antiseptic decamethoxine (1.10-decamethylene-bis-n, ndimethylmen-tihoxycarbonylmethyl, ammonium dichloride) and 2-[(1S, 6R)3,5-Dibromo-1,6-dihydroxy-4methoxycyclohexa-2,4-dien-1-yl] acetonitrile (Aeroplysinin 1) was determined by serial dilutions of antibiotics in a liquid nutrient medium [14]
Propionibacterum acnes cultures were isolated in 15 patients (30.0 %)
Summary
In the context of the global spread of the phenomenon of polyresistance of microorganisms to antimicrobials, the urgency of finding treatments for diseases of microbial etiology is constantly growing. Despite the development of the latest medical technologies, the number of skin diseases of microbial origin is growing. One of the most common dermatoses that significantly impairs the quality of life of patients is acne. Acne (acne vulgaris, acne) affects more than 85 % of people aged 12 to years, 8 % of people aged to years and 3 % of people aged to 44 years. Since acne is most often localized on exposed areas of the skin (face and upper torso), patients develop psycho-emotional disorders due to low self-esteem and peculiarities of perception in the social environment [3, 11, 21]
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