Abstract
AbstractStudies of the properties of subtilisin, Novo type, immobilized on porous glass with the aid of hexamethylene diisocyanate were carried out. The immobilized proteinase preparation shows optimum activity at a pH value of 10.7 and at a temperature between 60–65°C. It was stable in a wider range of pH and temperature values than the native subtilisin. The KM values for hemoglobin and BAEE were 9.2 × 10−5 [M] and 139 × 10−5 [M], respectively. Under relatively non‐aqueous conditions, immobilised subtilisin was able to synthesize phenylacetic acid ethyl ester.
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