Abstract
The presence of γ-glutamyl lactamase in human liver, kidney, serum, and other tissues has been demonstrated. A method for the quantitative determination of this enzyme, using [γ- l- 14C]glutamyl-γ- l-glutamyl-α-naphthylamide as substrate, has been described. The terminal glutamyl group is converted to pyrrolidone carboxylic acid, which is separated from the reaction mixture by paper electrophoresis and estimated by liquid scintillation counting. The level of the enzyme in normal tissues and in selected pathological sera has been determined.
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