Abstract
Gold nanoparticles are attractive materials for use in biomedicine due to their unique properties. Increasing evidence suggests that several nanoparticles induce differentiation of human mesenchymal stem cells (hMSCs) into osteoblast and adipocyte. In this study, we hypothesized that chitosan‐conjugated gold nanoparticles promote osteogenic differentiation of hMSCs. In order to investigate promoted osteogenic differentiation of hMSCs and related signaling pathway, chitosan‐conjugated gold nanoparticles were added to an osteogenic induction medium for 7, 10, 14 and 21 days. For evaluation of osteogenic differentiation, alizarin red staining, alamar blue assay and quantitative real time PCR were performed. In order to examine specific signaling pathways, immunofluorescence and western blotting assay were performed. Our results demonstrate that nanoparticles increase the deposit of calcium content and the expression of marker genes related to the early stage of osteogenic differentiation in hMSCs at non‐toxic concentrations. These results indicate that nanoparticles promote osteogenesis through Wnt/β‐catenin signaling pathway and may be used as a promoting reagent for bone formation.Grant Funding Source: supported by the National Research Foundation of Korea (2012R1A2A2A01047151)
Published Version
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