Abstract
Experimental conditions have been defined to allow human umbilical vein endothelial cells to grow in the complete absence of serum. Endothelial cells maintained in fibronectin-precoated plastic dishes could be grown in medium RPMI-1640 supplemented with transferrin, insulin, human serum albumin (HSA) and epidermal growth factor (EGF) or fibroblast growth factor (FGF). Endothelial cell growth supplement (ECGS) stimulated the proliferation in the presence of EGF or FGF, but its presence is not absolutely required to obtain growth of endothelial cells in serum-free medium. Serum was only required for a short period to allow attachment and spreading of the cells after trypsinization. Endothelial cells grown to confluence in serum-free medium exhibited morphological characteristics comparable to cells cultured in the presence of human serum, secreted normal quantities of factor VIII-related antigen into the culture medium, and synthesize prostacyclin.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
