Abstract
46 cases of invasive adenocarcinoma of the prostate diagnosed between 1985 and 1986 were retrieved from the files of CGMC. These cases were reviewed by on of us (HP) and were all graded according to Gleason. Seven other cases were rejected as having tissue unsatisfactory for processing by flow cytometry. The 46 cases were formed into a single nuclear suspension using a modified Hedley technique and the propidium iodide nuclear staining was measured using a Coulter<sup>R</sup> Profile II flow cytometer with a 488nm air-cooled Argon laser. Each case had a minimum count of 5000 nuclei in the gated histogram FL3 V FL3Peak. The DNA histograms were assessed for DNA ploidy and S-phase fraction using the Multicycle<sup>R</sup> software on an IBM-compatible 386 computer with a maths coprocessor. In all cases a background fitting algorithm for sliced nuclei was applied. Of the 46 cases, 39 were DNA diploid, 2 aneuploid and 5 tetraploid. Mean s-phase fraction for the diploid cases was 13.0% with a median of 10.2%. Sixteen of the 3 9 diploid cases had SPF > 13.0% and 19 cases > 10.2%. For the aneuploid cases the mean and median were the same with 20.8%. For the tetraploid cases the mean SPF was 8.2% with a median of 14.4%.
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