Abstract
A perfused fluidized bed bioreactor packed with SIRAN porous glass spheres was used for the cultivation of an anchorage dependent recombinant BHK cell line producing human interleukin-2, constitutively. Cultivations were carried out with different medium formulations over 80 days. Maximal cell densities and product yield were maintained even when protein-free medium was perfused, as a result less than 10% cell wash out. Final cell densities of the order of 3.8·108 ml-1 intrasphere volume were attained while the interleukin-2 production rate was 0.45 mg d-1. The production rate showed a 1.9-fold decrease compared with an homogeneous stirred bubble-free aerated system. This result was in contrast to that achieved with hybridoma cell lines, where better performances with respect to productivity were obtained with the fluidized bed bioreactor.
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