Abstract
During comparison of microcarrier and suspension cultures with recombinant BHK cells a specific proteolytic activity appeared only when the cells grew in suspension. The protease was detected by its specificity in cleaving the recombinant product into smaller peptides. Approximately one-third of the total amount of the product was inactivated as estimated by Western-blot analysis. No proteolytic activity was recognized when using an anchorage dependent state of growth which was investigated by several fermentations with different microcarrier types.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
