Abstract
Bioethanol from Rimau Gerga Lebong (RGL) orange waste is one of the solution to overcome fuel oil problem. The aim of this research is to get the type of microorganisms and fermentation time that produce the highest ethanol from RGL orange waste. The research method used was Randomized Block Design (RBD) of two factors, namely type of microorganisms (Trichoderma viride, Saccharomyces cerevisiae, and Trichoderma viride + Saccharomyces cerevisiae) and fermentation time (3 days, 5 days, and 7 days. Within the three type of microorganisms with variations of fermentation time showed that the pH was able to carry out the fermentation process smoothly. The highest total dissolved solids were in the type of Trichoderma viride 3 days and 5 days, and the type of mix of microorganisms on the 3rd day. The highest ethanol content is in the type of Sachharomyces cerevisiae for 7 days. ANOVA result showed that the interaction between two treatments on the total dissolved solids experienced significant differences, so it continue with the DMRT test at a significant level of 0.5%. However, it is different from the results of ANOVA on ethanol content which showed that there were significant differences between ethanol content and types of microorganisms, but there was no significant difference on fermentation time
Highlights
Orange is one of the fruits which containing cellulose
This study aims to obtain the type of microorganisms and fermentation time that can produce the highest ethanol from bioethanol manufacture of Rimau Gerga Lebong (RGL) orange waste
All the types of microorganisms which used in the https://doi.org/10.10 51/matecconf /201824804004 fermentation process shows that the longer the fermentation the more the pH decreased, as shown in figure 1
Summary
Orange is one of the fruits which containing cellulose. Orange juice contains cellulose around 0,52 gram per 50 ml and cell wall of orange fruit contain cellulose of 25-35% [1], orange peel contains cellulose of 5.36% [2]. [3] Reported that orange fruit yield ethanol levels of 9.75% on acid hydrolysis. Orange juice contains cellulose around 0,52 gram per 50 ml and cell wall of orange fruit contain cellulose of 25-35% [1], orange peel contains cellulose of 5.36% [2]. Rimau Gerga Lebong (RGL) orange is originally orange species, which developed in Bengkulu Province. This orange becomes the preeminent commodity of Lebong Regency [5]. RGL orange have competitive advantages, namely the colour of yellow-orange fruit, fruitful throughout the year, large fruit size, high https://doi.org/10.10 51/matecconf /201824804004 fruit juice and has a good market potential as the demand for orange RGL reached 100 tons/month, and contains 11.4 grams of carbohydrates [6]
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