The primary structure of aspartate transfer ribonucleic acid from brewers yeast: I. Complete digestion with pancreatic ribonuclease and T 1 ribonuclease

Abstract

Highly purified tRNA Asp from brewer's yeast prepared by countercurrent distribution followed by column chromatography has been completely digested with pancreatic ribonuclease and with T 1 ribonuclease. The separation and identification of the products have been carried out either by column chromatography followed by high voltage electrophoresis (in the case of T 1 ribonuclease digestion) or by this second technique alone (in the case of pancreatic ribonuclease digestion). Analyses indicate that this tRNA is composed of 75 nucleotide residues including 8 minor nucleotides. This tRNA contains a pUp nucleotide at the 5′ terminal end, the G-T-Ψ-C sequence common to all tRNAs of known structure and the oligonucleotide G-C-C-A at the 3′ terminal end. The primary structure of this tRNA has been determined after partial digestion with pancreatic and T 1 ribonucleases as stated in the subsequent paper.