Abstract
Summary Brewer's yeast tRNA Arg III , 93–98 p. cent pure was prepared by countercurrent distribution followed by chromatography on a column of DEAE cellulose at 65° and by a reversed phase chromatography (RPC 1). It was completely digested with pancreatic ribonuclease and with T 1 ribonuclease. The separation and identification of the products were carried out by column chromatography followed by high voltage electrophoresis. The results of the analyses indicate that tRNA Arg III is in fact a mixture of at least two different subspecies, one of the differences being located in the 3′ terminal sequence. They both contain 12 minor nucleotides. A sequence of 21 nucleotides : A-C-U-xU-C-U-t 6 A-A-Ψ-C-A-G-A-A-G-A-hU-U-A-U-Gp has been deduced from the results obtained. The primary structure of the two tRNAs Arg III has been determined after partial digestion with pancreatic and T 1 ribonucleases as stated in the following paper.
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